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Numerical Aperture Formula

Calculate numerical aperture (NA) for optical fibers and microscope objectives.
Covers acceptance angle, light-gathering power, and resolution limit formulas.

The Formula

NA = n × sin(θ)

Numerical aperture describes how much light an optical system can collect. Higher NA means better resolution and brighter images, but shallower depth of field.

Variables

SymbolMeaning
NANumerical aperture (unitless, typically between 0 and 1.5)
nRefractive index of the medium between the lens and specimen
θHalf-angle of the maximum cone of light entering the lens

For optical fibers: NA = √(n₁² - n₂²), where n₁ is the core index and n₂ is the cladding index.

Example 1

A microscope objective in air has a half-angle of 45°. Find the NA.

n = 1.00 (air), θ = 45°

NA = 1.00 × sin(45°)

NA = 0.707

Example 2

An optical fiber has core index n₁ = 1.50 and cladding index n₂ = 1.46

NA = √(1.50² - 1.46²) = √(2.25 - 2.1316)

NA = √0.1184

NA ≈ 0.344

When to Use It

Use the numerical aperture formula when:

  • Choosing microscope objectives for the resolution you need
  • Designing optical fiber systems and calculating acceptance angles
  • Comparing the light-gathering power of different optical systems
  • Estimating the resolution limit of an imaging system

Key Notes

  • Minimum resolvable feature (Abbe's diffraction limit): d = λ / (2 × NA) — doubling NA halves the resolution limit; this is why oil-immersion objectives (n ≈ 1.515) resolve ~50% finer detail than air objectives of the same design
  • NA > 1.0 is achievable only in an immersion medium (water n=1.33, oil n=1.515) — dry air-based systems are fundamentally limited to NA < 1.0 by Snell's law
  • For optical fibers, the acceptance angle (full cone) = 2 × arcsin(NA) — light entering outside this cone escapes into the cladding and is not guided along the fiber
  • Depth of field is inversely proportional to NA² — high-NA objectives used in microscopy have extremely shallow depth of field, requiring precise focusing but enabling optical sectioning

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